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1337Welcome to the official site of IICBEE. Our goal is to to bring together Researchers, Scientists, Engineers, Scholars and Students in the areas of Engineering & Bio-sciences, and provides a forum for dissemination of results, new ideas, Research & development and practical experiments, which concentrate on both theory and practices, for the benefit of human Being and Society.
IICBEE-Chair

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WORKSHOP on CANCER CELL CULTURE & MTT ASSAY

WORKSHOP on CANCER CELL CULTURE & MTT ASSAY (Sept. 17, 2014)

CEBS-2014 is also sponsoring a WORKSHOP on CANCER CELL CULTURE & MTT ASSAY for all researchers in the fields of medical and biological sciences. This workshop is proposed for Sept. 17, 2014. If you like to register for the workshop then email at: info@iicbe.org  so that the registartion form can be provided to you. The additional fee details of the workshop are as under:

REGISTRATION FEE (only for CANCER CELL CULTURE & MTT ASSAY)

Early Conference Registration Fees 

(before August 20, 2014)

Late Conference Registration Fees

(After August 20, 2014)

-Author Delegates

100 USD

Author Delegates

150 USD

-Student Delegates

70 USD

Student Delegates

100 USD

 

Agenda of WORKSHOP on CANCER CELL CULTURE & MTT ASSAY 

 

9:15-9:30

Registration

9:30-10:30

Basis of cancer cell culture, laboratory set up including culture hood, CO 2 incubator, water bath and inverted microscope. Media preparation, Culturing a cell line

10:40-12:00

Inverted microscope, Cell viability, Counting of cells by hemocytometer

LUNCH

1:15-2:45

Properties of attached cells vs the cells in suspension, how to harvest cells. Making flask

2:45-3:00                                                Break

3:00-4:30

Cell freezing procedure. Trypsinize and splitting the attached cells, Cell count

4:30-5:30

MTT assay technique